CGI-58 facilitates lipolysis on lipid droplets but is not involved in the vesiculation of lipid droplets caused by hormonal stimulation
Y Tomohiro, O Naoto, M Emi, N Hiromi, U Kanki… - Journal of lipid …, 2007 - ASBMB
A lipid droplet (LD)-associated protein, perilipin, is a critical regulator of lipolysis in
adipocytes. We previously showed that Comparative Gene Identification-58 (CGI-58), a
product of the causal gene of Chanarin-Dorfman syndrome, interacts with perilipin on LDs.
In this study, we investigated the function of CGI-58 using RNA interference. Notably, CGI-58
knockdown caused an abnormal accumulation of LDs in both 3T3-L1 preadipocytes and
Hepa1 hepatoma cells. CGI-58 knockdown did not influence the differentiation of 3T3-L1 …
adipocytes. We previously showed that Comparative Gene Identification-58 (CGI-58), a
product of the causal gene of Chanarin-Dorfman syndrome, interacts with perilipin on LDs.
In this study, we investigated the function of CGI-58 using RNA interference. Notably, CGI-58
knockdown caused an abnormal accumulation of LDs in both 3T3-L1 preadipocytes and
Hepa1 hepatoma cells. CGI-58 knockdown did not influence the differentiation of 3T3-L1 …
